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reddottm2 far red nuclear stain  (Biotium)


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    Structured Review

    Biotium reddottm2 far red nuclear stain
    Reddottm2 Far Red Nuclear Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reddottm2+far+red+nuclear+stain/RedDot2/bio_rxiv__64898__2026__03__16__712092-52-23-27
    Average 95 stars, based on 164 article reviews
    reddottm2 far red nuclear stain - by Bioz Stars, 2026-09
    95/100 stars

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    Staining:

    Article Title: Physiologically relevant media are associated with overlapping metabolic responses in primary human hepatocytes and Huh7 cells
    Article Snippet: .. Coverslips were then washed twice with PBS and stained with HCS LipidTOXTM Red Neutral Lipid Stain (ThermoFisher, H34476) at 1:200 in PBS and RedDotTM2 Far-Red Nuclear Stain (Biotium, 40061) at 1:125 in PBS for 1 hour followed by two more washes with PBS and staining with Oregon GreenTM 488 Phalloidin (ThermoFisher, O7466) at 1:40 in PBS for 30mins. .. Coverslips were then washed twice with PBS and mounted onto a slide with VECTASHIELD Vibrance® Antifade Mounting Medium (Vector Laboratories, H-1700) before imaging with a confocal microscope (Bio-Rad, Radiance 2100).

    Article Title: Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
    Article Snippet: .. In this study, we used the following dyes and secondary antibodies: propidium iodide (PI, Molecular Probes, # P21493 ), SYTO TM 16 (designated SYTO 16, Thermo Fisher Scientific #S7578), DAPI (Dojindo Molecular Technologies #D0523), SYTOX TM Green (designated SYTOX-G, Thermo Fisher Scientific #S7020), BOBOTM-1 Iodide (462/481) (designated BOBO-1, Thermo Fisher Scientific #B3582), RedDotTM2 Far-Red Nuclear Stain (designated RedDot2, Biotium #40061), NeuroTraceTM 640/660 Deep-Red Fluorescent Nissl Stain (Thermo Fisher Scientific # N21483 ), eosin (Acid Red 87, Tokyo Chemical Industry/TCI #T0037), anti-NeuN (A488-conjugated, Merck Millipore MAB377X), anti-mouse secondary IgG (Thermo Fisher Scientific, # A11029 for Alexa 488, # A21203 for Alexa 594), anti-rabbit secondary IgG (Thermo Fisher Scientific # A21206 for Alexa 488, # A10040 for Alexa 546, # A21207 for Alexa 594, # A31573 for Alexa 647), and FabuLight TM AffiniPure Fc specific Fab Fragments [Jackson ImmunoResearch laboratories; anti-mouse IgG 1 (#115-547-185 for Alexa 488, #115-167-185 for Cy3, #115-587-185 for Alexa 594, #115-607-185 for Alexa 647), anti-mouse IgG 2a (#115-587-186 for Alexa 594, #115-607-186 for Alexa 647), anti-mouse IgG 2b (#115-587-187 for Alexa 594), anti-rat IgG 1 (#112-167-008 for Cy3, #112-587-008 for Alexa 594), anti-rabbit IgG (#111-167-008 for Cy3, #111-587-008 for Alexa 594, #111-607-008 for Alexa 647), and anti-goat IgG (#805-587-008 for Alexa 594)]. ..



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